兔抗PSMD12多克隆抗体

Background:

The 26S proteasome is a multicatalytic proteinase complex with a highly ordered structure composed of 2 complexes, a 20S core and a 19S regulator. The 20S core is composed of 4 rings of 28 non-identical subunits; 2 rings are composed of 7 alpha subunits and 2 rings are composed of 7 beta subunits. The 19S regulator is composed of a base, which contains 6 ATPase subunits and 2 non-ATPase subunits, and a lid, which contains up to 10 non-ATPase subunits. Proteasomes are distributed throughout eukaryotic cells at a high concentration and cleave peptides in an ATP/ubiquitin-dependent process in a non-lysosomal pathway. An essential function of a modified proteasome, the immunoproteasome, is the processing of class I MHC peptides. This gene encodes a non-ATPase subunit of the 19S regulator. A pseudogene has been identified on chromosome 3. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:

ELISA, WB, IHC

Name of antibody:

PSMD12

Immunogen:

Fusion protein of human PSMD12

Full name:

proteasome 26S subunit, non-ATPase 12

Synonyms:

p55; Rpn5; STISS

SwissProt:

O00232

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human lung cancer and Human tonsil

IHC Recommend dilution:

40-200

WB Predicted band size:

53 kDa

WB Positive control:

Rat heart tissue lysate

WB Recommended dilution:

500-2000