兔抗HLA-DMB多克隆抗体

Background:

HLA-DMB belongs to the HLA class II beta chain paralogues. This class II molecule is a heterodimer consisting of an alpha (DMA) and a beta (DMB) chain, both anchored in the membrane. It is located in intracellular vesicles. DM plays a central role in the peptide loading of MHC class II molecules by helping to release the CLIP (class II-associated invariant chain peptide) molecule from the peptide binding site. Class II molecules are expressed in antigen presenting cells (APC: B lymphocytes, dendritic cells, macrophages). The beta chain is approximately 26-28 kDa and its gene contains 6 exons. Exon one encodes the leader peptide, exons 2 and 3 encode the two extracellular domains, exon 4 encodes the transmembrane domain and exon 5 encodes the cytoplasmic tail.

Applications:

ELISA, WB, IHC

Name of antibody:

HLA-DMB

Immunogen:

Synthetic peptide of human HLA-DMB

Full name:

major histocompatibility complex, class II, DM beta

Synonyms:

RING7; D6S221E

SwissProt:

P28068

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human tonsil

IHC Recommend dilution:

25-100

WB Predicted band size:

29 kDa

WB Positive control:

Raji cell lysate

WB Recommended dilution:

200-1000