小鼠抗GAPDH单克隆抗体

Background:

This gene encodes tumor protein p53, which responds to diverse cellular stresses to regulate target genes that induce cell cycle arrest, apoptosis, senescence, DNA repair, or changes in metabolism. p53 protein is expressed at low level in normal cells and at a high level in a variety of transformed cell lines, where it's believed to contribute to transformation and malignancy. p53 is a DNA-binding protein containing transcription activation, DNA-binding, and oligomerization domains. It is postulated to bind to a p53-binding site and activate expression of downstream genes that inhibit growth and/or invasion, and thus function as a tumor suppressor. Mutants of p53 that frequently occur in a number of different human cancers fail to bind the consensus DNA binding site, and hence cause the loss of tumor suppressor activity. Alterations of this gene occur not only as somatic mutations in human malignancies, but also as germline mutations in some cancer-prone families with Li-Fraumeni syndrome. Multiple p53 variants due to alternative promoters and multiple alternative splicing have been found. These variants encode distinct isoforms, which can regulate p53 transcriptional activity.

Applications:

WB, IHC

Name of antibody:

TP53

Immunogen:

Fusion protein of human TP53

Full name:

tumor protein p53

Synonyms:

P53, BCC7, LFS1, TRP53

SwissProt:

P04637

IHC positive control:

Human esophagus cancer

IHC Recommend dilution:

100-500

WB Predicted band size:

44 kDa

WB Positive control:

231, 293T cell lysates

WB Recommended dilution:

1000-5000