小鼠抗CHAF1B单克隆抗体

Background:

Chromatin assembly factor I (CAF-I) is required for the assembly of histone octamers onto newly-replicated DNA. CAF-I is composed of three protein subunits, p50, p60, and p150. The protein encoded by this gene corresponds to the p60 subunit and is required for chromatin assembly after replication. The encoded protein is differentially phosphorylated in a cell cycle-dependent manner. In addition, it is normally found in the nucleus except during mitosis, when it is released into the cytoplasm. This protein is a member of the WD-repeat HIR1 family and may also be involved in DNA repair. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC, IF

Name of antibody:

CHAF1B

Immunogen:

Fusion protein of human CHAF1B

Full name:

chromatin assembly factor 1, subunit B (p60) (CHAF1B)

Synonyms:

CAF-1; CAF-IP60; CAF1; CAF1A; CAF1P60; MPHOSPH7; MPP7

SwissProt:

Q13112

IHC positive control:

carcinoma of human prostate tissue and human lymph node tissue; human lymphoma tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

61 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, PC12, MCF-7 cell lysates

WB Recommended dilution:

200-1000