小鼠抗CDK1单克隆抗体

Background:

The protein encoded by this gene is a member of the Ser/Thr protein kinase family. This protein is a catalytic subunit of the highly conserved protein kinase complex known as M-phase promoting factor (MPF), which is essential for G1/S and G2/M phase transitions of eukaryotic cell cycle. Mitotic cyclins stably associate with this protein and function as regulatory subunits. The kinase activity of this protein is controlled by cyclin accumulation and destruction through the cell cycle. The phosphorylation and dephosphorylation of this protein also play important regulatory roles in cell cycle control. Alternatively spliced transcript variants encoding different isoforms have been found for this gene.

Applications:

WB, IF

Name of antibody:

CDK1

Immunogen:

Fusion protein of human CDK1

Full name:

cyclin dependent kinase 1

Synonyms:

CDC2; CDC28A; P34CDC2

SwissProt:

P06493

WB Predicted band size:

34 kDa

WB Positive control:

Jurkat, A549, K562 and Hela cell lysates

WB Recommended dilution:

200-1000

IF positive control:

Hela cells

IF Recommend dilution:

10-50