小鼠抗CDC123单克隆抗体

Background:

The eukaryotic cell division cycle consists of a number of gene-controlled sequences that involve cyclin dependent kinases (Cdks) and cell division control (Cdc) proteins. Cdc123 (Cell division cycle protein 123), also known as D123, is a 336 amino acid cytoplasmic protein that is involved in cell cycle control. Widely expressed with high expression in thymus, spleen, ovary, testis, small intestine and leukocytes, Cdc123 functions to destabilize Chfr (checkpoint with forkhead and ring finger domain) proteins which, when accumulated, block the G to S phase transition. Cdc123 prevents the Chfr proteins from collecting in the cell, thereby allowing the cell to enter the S phase. Due to its role in cell cycle control, Cdc123 is thought to be a basal marker for luminal breast cancers.

Applications:

WB, IHC, IF

Name of antibody:

CDC123

Immunogen:

Fusion protein of human CDC123

Full name:

cell division cycle 123 (CDC123)

Synonyms:

C10orf7; D123

SwissProt:

O75794

IHC positive control:

adenocarcinoma of human endometrium tissue and adenocarcinoma of human colon tissue; human kidney tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

39 kDa

WB Positive control:

HepG2, Hela, A549, COS7, Jurkat, MDCK, MCF-7 cell lysates

WB Recommended dilution:

500-2000