小鼠抗ARFGAP1单克隆抗体

Background:

The protein encoded by this gene is a GTPase-activating protein (GAP) which associates with the Golgi apparatus and which interacts with ADP-ribosylation factor 1 (ARF1). The encoded protein promotes hydrolysis of ARF1-bound GTP and is required for the dissociation of coat proteins from Golgi-derived membranes and vesicles. Dissociation of the coat proteins is required for the fusion of these vesicles with target compartments. The activity of this protein is stimulated by phosphoinosides and inhibited by phosphatidylcholine. Two transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq].

Applications:

WB, IHC, IF, FC

Name of antibody:

ARFGAP1

Immunogen:

Fusion protein of human ARFGAP1

Full name:

ADP-ribosylation factor GTPase activating protein 1 (ARFGAP1), transcript variant 2

Synonyms:

ARF1GAP; HRIHFB2281

SwissProt:

Q8N6T3

IHC positive control:

human kidney tissue and human liver tissue; human pancreas tissue and carcinoma of human thyroid tissue

IHC Recommend dilution:

30-150

WB Predicted band size:

45 kDa

WB Positive control:

HepG2, Hela, HT-29, A549, COS7, Jurkat, MCF-7 cell lysates

WB Recommended dilution:

1000-5000