兔抗STX5多克隆抗体

Background:

The membrane protein syntaxin 5 (STX5) is a key component of soluble N-ethylmaleimide-sensitive factor attachment protein (SNAP) receptor (SNARE) complexes that regulate cellular protein transport, vesicle docking, and membrane fusion. Syntaxin 5 protein is found as a 42 kDa ("long") protein localized to the Golgi complex and endoplasmic reticulum, and a “short” 35 kDa isoform localized primarily to the Golgi. Formation of the syntaxin 5 SNARE complex, which also includes proteins Sec22B, Bet1, GOSR1, GOSR2, and Ykt6, allows for regulation of ER-to-Golgi transport, intra-Golgi transport, and endosome-to-Golgi retrograde transport. Research studies indicate that the syntaxin 5 SNARE complex also plays an essential role in autophagy following autophagosome formation. Intracellular protein transport mediated by the syntaxin 5 complex is required for transport and localized activity of lysosomal proteases. The experimental reduction or deletion of syntaxin 5 complex components results in non-functional lysosomes and accumulation of autophagosomes.

Applications:

ELISA, WB, IHC

Name of antibody:

STX5

Immunogen:

Fusion protein of human STX5

Full name:

syntaxin 5

Synonyms:

SED5; STX5A

SwissProt:

Q13190

ELISA Recommended dilution:

5000-10000

IHC positive control:

Human liver cancer

IHC Recommend dilution:

50-200

WB Predicted band size:

40 kDa

WB Positive control:

HepG2, A431 and Raji cell lysates

WB Recommended dilution:

500-2000