兔抗NFKB1 (Phospho-Ser927)多克隆抗体

Background:

NF-kappa-B is a pleiotropic transcription factor which is present in almost all cell types and is involved in many biological processed such as inflammation, immunity, differentiation, cell growth, tumorigenesis and apoptosis. NF-kappa-B is a homo- or heterodimeric complex formed by the Rel-like domain-containing proteins RELA/p65, RELB, NFKB1/p105, NFKB1/p50, REL and NFKB2/p52 and the heterodimeric p65-p50 complex appears to be most abundant one. The dimers bind at kappa-B sites in the DNA of their target genes and the individual dimers have distinct preferences for different kappa-B sites that they can bind with distinguishable affinity and specificity. Different dimer combinations act as transcriptional activators or repressors, respectively.

Applications:

WB, IHC, IF

Name of antibody:

NFKB1 (Phospho-Ser927)

Immunogen:

Synthetic peptide of human NFKB1 (Phospho-Ser927)

Full name:

nuclear factor of kappa light polypeptide gene enhancer in B-cells 1 (Phospho-Ser927)

Synonyms：

p50; KBF1; p105; EBP-1; NF-kB1; NFKB-p50; NFkappaB; NF-kappaB; NFKB-p105; NF-kappa-B

SwissProt:

P19838

IHC positive control:

Human breast carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

120 kDa

WB Positive control:

HL60 cells treated with TNF-α

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200