Background: |
Fluorescein isothiocyanate (FITC) is a derivative of fluorescein used in wide-ranging applications including flow cytometry. FITC is the original fluorescein molecule functionalized with an isothiocyanate reactive group (-N=C=S), replacing a hydrogen atom on the bottom ring of the structure. This derivative is reactive towards nucleophiles including amine and sulfhydryl groups on proteins. |
Applications: |
ELISA |
Name of antibody: |
FITC |
Immunogen: |
KLH conjugated FITC |
Full name: |
Fluorescein isothiocyanate |
ELISA Recommended dilution: |
5000-10000 |