兔抗MAPK8 MAPK9 MAPK10(phospho-Thr183 Tyr185)多克隆抗体

Background:

Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as JUN, JDP2 and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells By similarity. Phosphorylates heat shock factor protein 4 (HSF4). /Responds to activation by environmental stress and pro-inflammatory cytokines by phosphorylating a number of transcription factors, primarily components of AP-1 such as c-Jun and ATF2 and thus regulates AP-1 transcriptional activity. In T-cells, JNK1 and JNK2 are required for polarized differentiation of T-helper cells into Th1 cells. JNK2 isoforms display different binding patterns: alpha-1 and alpha-2 preferentially bind to c-Jun, whereas beta-1 and beta-2 bind to ATF2. However, there is no correlation between binding and phosphorylation, which is achieved at about the same efficiency by all isoforms. JUNB is not a substrate for JNK2 alpha-2, and JUND binds only weakly to it.

Applications:

WB, IF

Name of antibody:

MAPK8/MAPK9/MAPK10 (phospho-Thr183/Tyr185)

Immunogen:

Synthetic peptide of human MAPK8/MAPK9/MAPK10 (phospho-Thr183/Tyr185)

Full name:

MAPK8/MAPK9/MAPK10 (phospho-Thr183/Tyr185)

Synonyms：

Stress-activated protein kinase JNK1 ; c-Jun N-terminal kinase 1; JNK-46

SwissProt:

P45983/P45984/P53779

WB Predicted band size:

46 kDa; 54 kDa

WB Positive control:

C6 cells treated with anisomycin

WB Recommended dilution:

500-1000

IF Positive control:

Hela cells

IF Recommended dilution

100-200