兔抗CHUK (Phospho-Thr23)多克隆抗体

Background:

Acts as part of the IKK complex in the conventional pathway of NF-kappa-B activation and phosphorylates inhibitors of NF-kappa-B thus leading to the dissociation of the inhibitor/NF-kappa-B complex and ultimately the degradation of the inhibitor. As part of the non-canonical pathway of NF-kappa-B activation, the MAP3K14-activated CHUK/IKKA homodimer phosphorylates NFKB2/p100 associated with RelB, inducing its proteolytic processing to NFKB2/p52 and the formation of NF-kappa-B RelB-p52 complexes. Also phosphorylates NCOA3. Phosphorylates 'Ser-10' of histone H3 at NF-kappa-B-regulated promoters during inflammatory responses triggered by cytokines.

Applications:

WB, IHC

Name of antibody:

CHUK (Phospho-Thr23)

Immunogen:

Synthetic peptide of human CHUK (Phospho-Thr23)

Full name:

conserved helix-loop-helix ubiquitous kinase (Phospho-Thr23)

Synonyms：

IKK1; IKKA; IKBKA; TCF16; NFKBIKA; IKK-alpha

SwissProt:

O15111

IHC positive control:

Human colon carcinoma

IHC Recommend dilution:

50-100

WB Predicted band size:

85 kDa

WB Positive control:

293 cells untreated or treated with TNF

WB Recommended dilution:

500-1000