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021-54845833/15800441009
标 记 物： Unconjugate
克隆类型： mouse monoclonal
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Background: |
The 6 X histidine motif is used as a tag on many recombinant proteins to facilitate purification.It can then be purified using immobilized metal ions,traditionally nickel,in a procedure termed immobilized-metal affinity chromatography.Further analysis of the protein can be achieved using an antibody specific to the 6 X histidine in techniques such as western blotting.The antibody recognizes the His-tag fused to targeted proteins in transfected or transformed cells. |
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Applications: |
WB, IF, IP |
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Name of antibody: |
6×His Tag |
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Immunogen: |
Synthetic peptide conjugated with KLH |
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Full name: |
HHHHHH Tag |
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Synonyms: |
6 His epitope tag |
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WB Predicted band size: |
50 kDa |
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WB Positive control: |
Fusion proteins containing the His Tag |
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WB Recommended dilution: |
1000-5000 |
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IF Positive control: |
3T3 cells |
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IF Recommended dilution: |
100-500 |
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IP Recommended dilution: |
100-200 |
