小鼠抗NR0B2单克隆抗体

Background:

The protein encoded by this gene is an unusual orphan receptor that contains a putative ligand-binding domain but lacks a conventional DNA-binding domain. The gene product is a member of the nuclear hormone receptor family, a group of transcription factors regulated by small hydrophobic hormones, a subset of which do not have known ligands and are referred to as orphan nuclear receptors. The protein has been shown to interact with retinoid and thyroid hormone receptors, inhibiting their ligand-dependent transcriptional activation. In addition, interaction with estrogen receptors has been demonstrated, leading to inhibition of function. Studies suggest that the protein represses nuclear hormone receptor-mediated transactivation via two separate steps: competition with coactivators and the direct effects of its transcriptional repressor function. [provided by RefSeq, Jul 2008].

Applications:

WB, IHC

Name of antibody:

NR0B2

Immunogen:

Fusion protein of human NR0B2

Full name:

nuclear receptor subfamily 0, group B, member 2 (NR0B2)

Synonyms:

SHP; SHP1

SwissProt:

Q15466

IHC positive control:

adenocarcinoma of human breast tissue and human pancreas tissue; human lymphoma tissue and human tonsil tissue

IHC Recommend dilution:

25-100

WB Predicted band size:

28 kDa

WB Positive control:

HepG2, Hela, SVT2, A549, COS7, Jurkat, MDCK, MCF7 cell lysates

WB Recommended dilution:

500-2000